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KMID : 0903519990420010006
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1999 Volume.42 No. 1 p.6 ~ p.11
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The study on isolation of fibrinolytic bacteria from soybean paste
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Eo Gi-Soung
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Abstract
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The bacteria which could hydrolyze the fibrin produced through the blood coagulation mechanism in the human body, were isolated from soybean paste. The KDO-13 strain was selected among the isolated bacteria as the best strain for fibrinolytic activity. It was spore forming and Gram positive. C_(15:0) anteiso fatty acid, C_(15:0) iso fatty acid and C_(15:0) anteiso fatty acid were 47.7, 13.5 and 13.6%, respectively as major component among its cellular fatty acid composition. It showed the similarity of 57.7%, compared with standard strain. It was thus identified to be Bacillus atrophaeus according to Bergey´s manual of systematic bacteriology and its fatty acid profiles of gas chromatography. The optimum culture temperature and pH were 37℃ and 6 for the production of fibrinolytic enzyme by Bacillus atrophaeus KDO-13.
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